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Journal / Cancer Research / 在全融合微流体平台中增强患者衍生异种移植物的外体 3D…
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JoVE Journal Cancer Research
在全融合微流体平台中增强患者衍生异种移植物的外体 3D 水凝胶培养物的生存能力
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在全融合微流体平台中增强患者衍生异种移植物的外体 3D 水凝胶培养物的生存能力

Article DOI: 10.3791/60872-v • 12:12 min • December 5th, 2020
December 5th, 2020 •
1, 2, 3, 4, 1,5,6, 1,7, 2, 3, 1,5,6
1Department of BioSciences, Rice University, 2Mimetas US Inc, 3Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, 4Mimetas B.V., 5Department of Bioengineering, Rice University, 6Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, 7Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center

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该协议演示了启用患者衍生异种移植 （PDX） 的扩展体外培养的方法。通过直接去除不可行的单细胞，一步提高三维水凝胶中多细胞聚类培养的整体生存能力。辅助步骤演示了在全融合微流体平台中 PDX 培养的最佳实践。

Tags

癌症研究， 问题 166， 3D 培养， 水凝胶， 微流体， 患者衍生异种移植， 前列腺癌， 体外癌模型， 高通量药物筛查， 灌注
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