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Journal / Cancer Research / Förbättrad livskraft för Ex vivo 3D Hydrogel…
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Cancer Research

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JoVE Journal Cancer Research
Förbättrad livskraft för Ex vivo 3D Hydrogel Kulturer av patient-derived Xenografts i en Perfused mikrofluidisk plattform
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Förbättrad livskraft för Ex vivo 3D Hydrogel Kulturer av patient-derived Xenografts i en Perfused mikrofluidisk plattform

Article DOI: 10.3791/60872-v • 12:12 min • December 5th, 2020
December 5th, 2020 •
1, 2, 3, 4, 1,5,6, 1,7, 2, 3, 1,5,6
1Department of BioSciences, Rice University, 2Mimetas US Inc, 3Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, 4Mimetas B.V., 5Department of Bioengineering, Rice University, 6Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, 7Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center

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Detta protokoll demonstrerar metoder för att möjliggöra utökad in vitro-kultur av patient-härledda xenografts (PDX). Ett steg förstärker den totala livskraften hos flercelliga klusterkulturer i 3D-hydrogeler, genom okomplicerat avlägsnande av icke-livskraftiga enstaka celler. Ett sekundärt steg visar bästa praxis för PDX-kultur i en perfused mikrofluidisk plattform.

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Cancerforskning 3D-kultur hydrogeler mikrofluidik patienthärledda xenografts prostatacancer in vitro-modeller cancer hög genomströmning läkemedelsscreening perfusion
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