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Journal / Developmental Biology / Enkel litografifri mikromønster med én celle ved…
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Developmental Biology

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JoVE Journal Developmental Biology
Enkel litografifri mikromønster med én celle ved hjelp av laserkuttede sjablonger
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Enkel litografifri mikromønster med én celle ved hjelp av laserkuttede sjablonger

Article DOI: 10.3791/60888-v • 08:59 min • April 3rd, 2020
April 3rd, 2020 •
*1,2,3, *1,2,3, *1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3,4,5
1Stanford Cardiovascular Institute, Stanford University School of Medicine, 2Department of Medicine, Division of Cardiovascular Medicine, Stanford University, 3Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 4Department of Medicine III, University Hospital Heidelberg, 5German Centre for Cardiovascular Research (DZHK), Partner Site Heidelberg/Mannheim
* These authors contributed equally

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Denne protokollen introduserer en litografifri mikromønstermetode som er enkel og tilgjengelig for de med begrenset bioingeniørbakgrunn. Denne metoden benytter tilpassede laserkuttede sjablonger til mikromønster ekstracellulære matriseproteiner i en form av interesse for modulerende cellemorfologier. Prosedyren for mikromønster er demonstrert ved hjelp av indusert pluripotent stamcelle avledet kardiomyocytter.

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Utviklingsbiologi Utgave 158 mikromønster for én celle sjablong cellemorfologi litografifri iPSC-CM hydrogel
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