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Journal / Immunology and Infection / Metal-Begrænset vækst af Neisseria gonorrée for…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Metal-Begrænset vækst af Neisseria gonorrée for karakterisering af metal-responsive gener og metal erhvervelse fra Host Ligands
Neutrophils Screening Assay Using Flow Cytometry
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High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
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High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
 
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Metal-Begrænset vækst af Neisseria gonorrée for karakterisering af metal-responsive gener og metal erhvervelse fra Host Ligands

Article DOI: 10.3791/60903-v • 07:26 min • March 4th, 2020
March 4th, 2020 •
1, 1
1Institute for Biomedical Sciences, Georgia State University

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Vi beskriver her en metode til vækst af Neisseria gonorrée i metal-begrænset flydende medium for at lette ekspressionen af gener for metaloptagelse. Vi skitserer også downstream eksperimenter for at karakterisere fænotype af gonococci dyrket under disse betingelser. Disse metoder kan tilpasses til at være egnet til karakterisering af metal-responsive gener i andre bakterier.

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Immunologi og infektion Neisseria gonorrée metaller definerede medier vækstanalyser bindende analyser bakterielle patogener
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