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JoVE Journal
Biology

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成人心脏纤维细胞和肌纤维细胞的分离与表征
 
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成人心脏纤维细胞和肌纤维细胞的分离与表征

Article DOI: 10.3791/60909-v 10:45 min March 12th, 2020
March 12th, 2020

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

获得成纤维细胞的纯种群对于研究其在伤口修复和纤维化中的作用至关重要。这里描述的是一种详细的方法,从未受伤和受伤的小鼠心脏中分离成纤维细胞和肌纤维细胞,然后通过免疫荧光、RTPCR、荧光辅助细胞分拣和胶原蛋白凝胶收缩。

Tags

生物学, 问题 157, 隔离, βSMA, 荧光活性细胞分拣, 成纤维细胞, 肌纤维细胞, 胶原蛋白凝胶, 免疫荧光, MEFSK4
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