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Journal / Biochemistry / Impronta proteica radicale in vivo Hydroxyl per…
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Biochemistry

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JoVE Journal Biochemistry
Impronta proteica radicale in vivo Hydroxyl per lo studio delle interazioni proteiche in Caenorhabditis elegans
Polysome Profiling without Gradient Makers or Fractionation Systems
Journal (Biochemistry)
Polysome Profiling without Gradient Makers or Fractionation Systems
Identification of Functional Protein Regions Through Chimeric Protein Construction
Journal (Biochemistry)
Identification of Functional Protein Regions Through Chimeric Protein Construction
 
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Impronta proteica radicale in vivo Hydroxyl per lo studio delle interazioni proteiche in Caenorhabditis elegans

Article DOI: 10.3791/60910-v • 08:00 min • April 1st, 2020
April 1st, 2020 •
1, 1
1Department of Pharmaceutical Sciences, University of Maryland

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L'ossidazione fotochimica veloce in vivo delle proteine (IV-FPOP) è una tecnica di impronta proteica radicale idrossile che consente di mappare la struttura delle proteine nel loro ambiente nativo. Questo protocollo descrive l'assemblaggio e l'impostazione del sistema di flusso microfluidico IV-FPOP.

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Biochimica Numero 158 Caenorhabditis elegans impronta delle proteine radicali idrossili FPOP spettrometria di massa in vivo proteomica
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