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JoVE Journal
Biochemistry

This content is Open Access.

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans In Vi
 
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In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans In Vi

Article DOI: 10.3791/60910-v 08:00 min April 1st, 2020
April 1st, 2020

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Summary

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In vivo snabb fotokemisk oxidation av proteiner (IV-FPOP) är en hydroxyl radikal protein footprinting teknik som möjliggör kartläggning av proteinstruktur i sin inhemska miljö. Detta protokoll beskriver montering och upplägg av IV-FPOP mikrofluidiska flödessystem.

Tags

Biokemi Utgåva 158 Caenorhabditis elegans hydroxylradikal proteinavtryck FPOP masspektrometri in vivo proteomik
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