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Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
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Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments

Article DOI: 10.3791/60912-v • 09:05 min • February 9th, 2020
February 9th, 2020 •
1, 1,2, 1,3, 1, 1,2, 1
1Sorbonne Université, Inserm, CNRS, UMR7225, Institut du Cerveau et de la Moelle épinière, ICM, 2Assistance Publique-Hôpitaux de Paris, GH Pitié Salpêtrière, 3Ecole Supérieure des Techniques de Biologie Appliquée

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Herein, we display an efficient method for the purification of oligodendrocytes and production of oligodendrocyte-conditioned medium that can be used for co-culture experiments.

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Oligodendrocytes Co-culture Experiments Oligodendrocyte-conditioned Medium Purification Culture Myelination Process Shaking Technique High Quantity Neurons Multiple Sclerosis Demyelination Central Nervous System Remyelination Intrinsic Mechanisms Developments And Biology Of Oligodendrocytes Neuronal Physiology Forceps Surgical Scissors Incision Skull Brain Midline Surgical Spoon Petri Dish
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