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Journal / Immunology and Infection / Aislamiento de células mononucleares tonitarias…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Aislamiento de células mononucleares tonitarias para estudiar las respuestas inmunitarias innatas ex vivo en un tejido linfático mucoso humano
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Journal (Immunology and Infection)
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Journal (Immunology and Infection)
Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
 
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Aislamiento de células mononucleares tonitarias para estudiar las respuestas inmunitarias innatas ex vivo en un tejido linfático mucoso humano

Article DOI: 10.3791/60914-v • 07:38 min • June 14th, 2020
June 14th, 2020 •
1,2,3,4, 2,3,4, 4,5, *6, *2,3,4
1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin
* These authors contributed equally

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En el presente protocolo, explicamos cómo procesar y cultivar fácilmente células mononucleares amídricas de seres humanos sanos sometidos a amigdalectomía quirúrgica parcial para estudiar las respuestas inmunitarias innatas tras la activación, imitando la infección viral en los tejidos de la mucosa.

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Inmunología e infección Número 160 tejido linfoide asociado a la mucosa amígdala célula mononuclear tonitaria inmunidad cultivo celular ex vivo citoquina interacción huésped-patógeno
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