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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolamento delle cellule mononucleari del Tonsillar per studiare le risposte immunitarie innate Ex Vivo in un tessuto linfoide mucosale umano
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Isolamento delle cellule mononucleari del Tonsillar per studiare le risposte immunitarie innate Ex Vivo in un tessuto linfoide mucosale umano

Article DOI: 10.3791/60914-v • 07:38 min • June 14th, 2020
June 14th, 2020 •
1,2,3,4, 2,3,4, 4,5, *6, *2,3,4
1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin
* These authors contributed equally

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Nel protocollo attuale, spieghiamo come elaborare e coltura facilmente le cellule mononucleari tonsillare da esseri umani sani sottoposti a tonsillectomia chirurgica parziale per studiare le risposte immunitarie innate dopo l'attivazione, imitando l'infezione virale nei tessuti mucosali.

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Immunologia e infezione numero 160 tessuto linfoide associato alla mucosa tonsilla cellula mononucleare tonsillaria immunità coltura di cellule ex vivo citochina interazione ospite-patogeno
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