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Journal / Immunology and Infection / Isolering av tonsillar mononukleære celler for å…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering av tonsillar mononukleære celler for å studere ex vivo medfødte immunresponser i et humant slimhinnelymfoid vev
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Isolering av tonsillar mononukleære celler for å studere ex vivo medfødte immunresponser i et humant slimhinnelymfoid vev

Article DOI: 10.3791/60914-v • 07:38 min • June 14th, 2020
June 14th, 2020 •
1,2,3,4, 2,3,4, 4,5, *6, *2,3,4
1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin
* These authors contributed equally

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I den nåværende protokollen forklarer vi hvordan man enkelt kan behandle og kultur tonsillar mononukleære celler fra friske mennesker som gjennomgår delvis kirurgisk mandlene for å studere medfødte immunresponser ved aktivering, etterligne virusinfeksjon i slimhinnevev.

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Immunologi og infeksjon utgave 160 slimhinne-assosiert lymfoid vev mandlene tonsillar mononukleær celle immunitet ex vivo celle kultur cytokin host-patogen interaksjon
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