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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolamento de células mononucleares tonsiláres para estudar respostas imunes inatas ex vivo em um tecido linfoide mucosal humano
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Isolamento de células mononucleares tonsiláres para estudar respostas imunes inatas ex vivo em um tecido linfoide mucosal humano

Article DOI: 10.3791/60914-v • 07:38 min • June 14th, 2020
June 14th, 2020 •
1,2,3,4, 2,3,4, 4,5, *6, *2,3,4
1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin
* These authors contributed equally

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No presente protocolo, explicamos como processar e cultivar facilmente células mononucleares tonsilares de humanos saudáveis submetidos a amidaltomia cirúrgica parcial para estudar respostas imunes inatas após a ativação, imitando infecção viral em tecidos mucosas.

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Imunologia e Infecção Questão 160 tecido linfoide associado à mucosa amígdala célula mononuclear amíillar imunidade cultura celular ex vivo citocina interação hospedeiro-patógeno
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