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JoVE Journal
Developmental Biology

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翻译核糖体亲和纯化(TRAP)以研究细胞类型特异性尺度下阿拉伯生物基塔利亚纳根发育
 
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翻译核糖体亲和纯化(TRAP)以研究细胞类型特异性尺度下阿拉伯生物基塔利亚纳根发育

Article DOI: 10.3791/60919-v 09:41 min May 14th, 2020
May 14th, 2020

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Summary

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Click here for the English version.

翻译核糖体亲和纯化(TRAP)提供了以最少的器官和组织处理来解剖发育方案的可能性。该协议从绿色荧光蛋白(GFP)标记的核糖体亚组细胞中产生高质量的RNA。下游分析工具(如 qRT-PCR 或 RNA-seq)可显示组织和细胞类型特定的表达特征。

Tags

发育生物学, 第 159 期, 阿拉伯生物, TRAP, TRAP-seq, 转体分析, 细胞类型特异性RNA-seq, 横向根形成, 内皮质分化
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