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Journal / Developmental Biology / RiboTag Immunudffældning i kimcellerne i den…
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Developmental Biology

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JoVE Journal Developmental Biology
RiboTag Immunudffældning i kimcellerne i den mandlige mus
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RiboTag Immunudffældning i kimcellerne i den mandlige mus

Article DOI: 10.3791/60927-v • 10:00 min • March 4th, 2020
March 4th, 2020 •
1, 1, 1
1Department of Animal Science, Rutgers University

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Her beskriver vi immunudfældning af ribosomer og tilhørende RNA fra udvalgte populationer af voksne mandlige musekimceller ved hjælp af RiboTag-systemet. Strategisk avl og omhyggelig immunudfældning resulterer i rene, reproducerbare resultater, der informerer om kimcellen stilbar og giver indsigt i mekanismerne i mutante fænotyper.

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Udviklingsbiologi cellespecifik mandlig kimcelle RNA RiboTag Ribosome oversættelse spermatogenese
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