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Journal / Developmental Biology / RiboTag immunoprecipitatie in de kiemcellen van…
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Developmental Biology

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JoVE Journal Developmental Biology
RiboTag immunoprecipitatie in de kiemcellen van de mannelijke muis
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RiboTag immunoprecipitatie in de kiemcellen van de mannelijke muis

Article DOI: 10.3791/60927-v • 10:00 min • March 4th, 2020
March 4th, 2020 •
1, 1, 1
1Department of Animal Science, Rutgers University

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Hier beschrijven we de immunopreciatie van riosomen en bijbehorend RNA uit geselecteerde populaties volwassen mannelijke muizenkiemcellen met behulp van het RiboTag-systeem. Strategische veredeling en zorgvuldige immunoneerslag resulteren in schone, reproduceerbare resultaten die informeren over het vertalen van de kiemcel en inzicht geven in de mechanismen van gemuteerde fenotypes.

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Ontwikkelingsbiologie nummer 157 celspecifieke mannelijke kiemcel RNA RiboTag Ribosoom vertaling spermatogenese
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