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Journal / Developmental Biology / Erkek Farenin Germ Hücrelerinde RiboTag…
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Developmental Biology

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JoVE Journal Developmental Biology
Erkek Farenin Germ Hücrelerinde RiboTag İmmünanyağış
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Erkek Farenin Germ Hücrelerinde RiboTag İmmünanyağış

Article DOI: 10.3791/60927-v • 10:00 min • March 4th, 2020
March 4th, 2020 •
1, 1, 1
1Department of Animal Science, Rutgers University

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Burada RiboTag sistemini kullanarak erişkin erkek fare mikrop hücrelerinin belirli popülasyonlarından ribozomların ve ilişkili RNA'nın immünopresipitesini tanımladık. Stratejik üreme ve dikkatli immünopresipitasyon, germ hücre translatomu hakkında bilgi veren ve mutant fenotiplerin mekanizmaları hakkında bilgi veren temiz, tekrarlanabilir sonuçlar la sonuçlanır.

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Gelişim Biyolojisi Sayı 157 hücreye özgü erkek germ hücresi RNA RiboTag Ribozom çeviri spermatogenez
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