Medicine
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兔子静脉介词模型模仿再血管化手术使用静脉移植评估动脉血压下的影响增生
Chapters
Summary May 15th, 2020
Please note that all translations are automatically generated.
Click here for the English version.
本议定书旨在通过将静脉置于动脉血压下，在使用静脉移植进行静脉移植手术后衰减静脉内血性增生的策略，从而在实验中产生静脉内质增生。
Transcript
我们的协议为静脉刺激增生领域的研究人员提供了一个简单、经济高效、可重复和临床相关的模型。我们的协议在经济上、临床上和技术上都是平衡的。因此，强烈建议那些希望将其体外结果应用于体内环境的研究人员。
我们相信，我们的协议可以应用于一个更大的动物模型，以开发新的治疗策略，在缺血性心脏和下肢疾病。我们的协议主要与心血管手术领域有关。然而，它也可用于解决动脉静脉管炎的退行性变化在患者的末期肾病。
局部麻醉后，预防性抗生素镇痛剂给药，用10%的波维多碘对手术现场进行消毒。并在麻醉的雄性日本白兔的颈椎区域用手术刀切口50至60毫米。模糊解剖皮下组织和筋膜，露出20至30毫米的血管段。
并使用 4-0 丝缝合线将暴露静脉的所有分支都缝合。在静脉的内脏和外静脉周围放置一个2-0丝状缝合，并在静脉的静脉壁上做一毫米切口。插入一个2-法国气球导管从切口向静脉的近端。
并在壶静脉的上将 2 - 0 的丝绸缝合线。用200微升的空气充气气球。并使用气球三次使静脉内皮去角质。
第三次通过后，将静脉的近端进行开盖，并在端点切开静脉。然后插入20量表静脉导管到收获的静脉从远向近向，并在近位点切割静脉。要将胡萝卜动脉与收获的血管进行插管，请露出20至30毫米的静脉胡萝卜动脉。
小心地将动脉与附近的静脉和神经分开。用 4 - 0 丝缝合将裸露静脉的所有分支都拉起。并静脉注射200国际单位每公斤肝素钠。
用手术橡胶夹夹住动脉的近端和近端。切开夹子之间的动脉将正常的盐水直接注入切开的胡萝卜动脉，以分散动脉。
将收获静脉放在动脉附近，以反向的端到端方式使血管变化。将静脉的近端对动脉的端端进行麻醉，放置两个 8-0 的锚缝聚丙烯在站点和对面的站点。在锚缝之间添加麻醉线的上侧。
翻转动脉和静脉移植倒置。并在动脉瘤线的剩余部分添加缝合。接下来，从静脉中取出静脉导管，并紧接静脉移植。
将胡萝卜动脉的灯对地开脱。观察静脉移植是否逐渐扩大。使用 8-0聚丙烯中断缝合，将静脉的端到动脉的近端。
并给动脉下灯，检查动脉瘤部位的出血。最后，用4-0丝线缝合将内胡萝卜状动脉装开，以模拟不良的径流状况，促进刺激性增生。然后用盐水清洁伤口。
用3-0聚加糖910层关闭切口。并允许兔子完全恢复监测之前，返回动物回家的笼子。在这个具有代表性的图像中，可以观察到静脉间穿手术后2周成功的刺激性增生。
在这里，可以观察到微RNA-145加载的多（乳酸共糖酸）纳米粒子对刺激性增生形态的治疗效果。事实上，使用含有聚（乳酸共糖酸）的微RNA进行治疗，可显著衰减内刺激性增生。此外，Ki-67（细胞增殖标记物）的免疫抑制显示微RNA-145治疗组内的Ki-67阳性细胞较少，表明血管平滑肌细胞的表型变化从不成熟的增殖状态到成熟的收缩状态。
我们协议的主要目标是实现植入移植物的可移植性。同样重要的是，要确认移植物在脱脱了胡萝卜动脉后会扩大。
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