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Journal / Bioengineering / Una piattaforma di microscopia multifoto…
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Bioengineering

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JoVE Journal Bioengineering
Una piattaforma di microscopia multifoto personalizzata per l'imaging dal vivo di Mouse Cornea e Conjunctiva
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Una piattaforma di microscopia multifoto personalizzata per l'imaging dal vivo di Mouse Cornea e Conjunctiva

Article DOI: 10.3791/60944-v • 06:53 min • May 17th, 2020
May 17th, 2020 •
1, 1, 2,3, *1,3,4,5, *6,7
1Department of Biomedical Engineering, National Taiwan University, 2Institute of Pharmacology, College of Medicine, National Taiwan University, 3Molecular Imaging Center, National Taiwan University, 4Department of Dermatology, National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 6Department of Ophthalmology, Chang Gung Memorial Hospital, 7College of Medicine, Chang Gung University
* These authors contributed equally

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Qui è presentata una piattaforma microscopica multifoto per l'imaging della superficie oculare del mouse vivo. Il mouse transgenico fluorescente consente la visualizzazione di nuclei cellulari, membrane cellulari, fibre nervose e capillari all'interno della superficie oculare. I segnali di seconda generazione armonica non lineari derivati da strutture collagenose forniscono immagini senza etichette per architetture tropiche.

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Bioingegneria Emissione 159 cornea congiuntiva superficie oculare microscopia multifoto MPM imaging intravitale topo transgenico
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