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JoVE Journal Bioengineering
En tilpasset multiphoton mikroskopi plattform for live imaging av mus cornea og conjunctiva
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En tilpasset multiphoton mikroskopi plattform for live imaging av mus cornea og conjunctiva

Article DOI: 10.3791/60944-v • 06:53 min • May 17th, 2020
May 17th, 2020 •
1, 1, 2,3, *1,3,4,5, *6,7
1Department of Biomedical Engineering, National Taiwan University, 2Institute of Pharmacology, College of Medicine, National Taiwan University, 3Molecular Imaging Center, National Taiwan University, 4Department of Dermatology, National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 6Department of Ophthalmology, Chang Gung Memorial Hospital, 7College of Medicine, Chang Gung University
* These authors contributed equally

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Presentert her er en multiphoton mikroskopisk plattform for levende mus okulær overflateavbildning. Fluorescerende transgen mus muliggjør visualisering av cellekjerner, cellemembraner, nervefibre og kapillærer innenfor den okulære overflaten. Ikke-lineære andre harmoniske generasjonssignaler avledet fra kollagenstrukturer gir etikettfri bildebehandling for stromal arkitekturer.

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Bioengineering Utgave 159 hornhinnen conjunctiva okulær overflate multiphoton mikroskopi MPM intravital avbildning transgen mus
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