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Journal / Genetics / Identifikation af forstærker-promotor kontakter i …
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Genetics

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JoVE Journal Genetics
Identifikation af forstærker-promotor kontakter i embryolede organer ved kvantitative kromosom kropsbygning Capture (4C)
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Identifikation af forstærker-promotor kontakter i embryolede organer ved kvantitative kromosom kropsbygning Capture (4C)

Article DOI: 10.3791/60960-v • 10:02 min • April 29th, 2020
April 29th, 2020 •
1,2,3, 1,2, 1,2, 1,2
1Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 2Universitat Pompeu Fabra, 3Vall d'Hebron Institute of Oncology

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Vi rapporterer om anvendelsen af kvantitativ kromosomkropsformation efterfulgt af sekvensering med høj gennemløb i embryoide kroppe, der stammer fra embryonale stamceller. Denne teknik gør det muligt at identificere og koronere kontakterne mellem formodede smagsforstærkere og promotorregioner for et givet gen under embryonal stamcelledifferentiering.

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Genetik udvikling transskription regulering embryoid krop afstamning specifikation forstærker promotor kromosom kropsbygning fange 4C
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