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Journal / Developmental Biology / Præparat af Drosophila Larve og Pupal Testikler…
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JoVE Journal Developmental Biology
Præparat af Drosophila Larve og Pupal Testikler til analyse af celledeling i Levende, Intakt væv
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Præparat af Drosophila Larve og Pupal Testikler til analyse af celledeling i Levende, Intakt væv

Article DOI: 10.3791/60961-v • 08:05 min • May 19th, 2020
May 19th, 2020 •
1, 1
1Department of Anatomy, Physiology, and Genetics, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences

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Målet med denne protokol er at analysere celledeling i intakt væv ved levende og fast celle mikroskopi ved hjælp af Drosophila meiotiske spermatocytter. Protokollen viser, hvordan man isolere hele, intakte testikler fra Drosophila larver og tidlig pupper, og hvordan man behandler og montere dem til mikroskopi.

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Udviklingsbiologi Drosophila spermatocyt testis mitose meiose celledeling mikrotubule spindel endoplasmisk reticulum
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