Chapters
Summary
Presented here is a protocol to evaluate the inhibition efficacy of chemical compounds against in vitro intracellular growth of Toxoplasma gondii using a luciferase-based growth assay. The technique is used to confirm inhibition specificity by genetic deletion of the corresponding target gene. The inhibition of LHVS against TgCPL protease is evaluated as an example.
Tags
Chemical Inhibitor Efficiency Intracellular Toxoplasma Gondii Growth Luciferase-based Growth Assay CRISPR-Cas9 Gene Deletion Drug Targets Parasite Doubling Time Reporter Protein 384 Well Microplates 1,536 Well Microplates Intracellular Microbial Pathogens High Throughput Screening Luciferase-based Toxoplasma Growth Assay Human Foreskin Fibroblasts Parasite B Suspension Phenol Red Free Medium Luciferase Substrate Solution
PLAYLIST
Antimicrobial Resistance
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Research • Immunology and InfectionVisualization of Bacterial Resistance using Fluorescent Antibiotic Probes -
Research • GeneticsQuantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach -
Research • Immunology and InfectionA Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment -
Research • BioengineeringBiosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria -
Research • EnvironmentScreening Foodstuffs for Class 1 Integrons and Gene Cassettes -
Research • BioengineeringHigh-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
Bacterial Pathogens
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Education • Core: BiologyBacterial Signaling -
Education • Lab BioMicrobial and Fungal Diversity- Concept -
Education • Environmental MicrobiologyIsolation of Fecal Bacteria from Water Samples by Filtration -
Education • Environmental MicrobiologyWater Quality Analysis via Indicator Organisms -
Education • MicrobiologyMicroscopy and Staining: Gram, Capsule, and Endospore Staining -
Research • Immunology and InfectionUse of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds -
Research • Immunology and InfectionLaboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Chemical Contaminants
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Research • EnvironmentLaboratory Estimation of Net Trophic Transfer Efficiencies of PCB Congeners to Lake Trout (Salvelinus namaycush) from Its Prey -
Research • BiochemistryStudy on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves -
Research • EnvironmentDetermination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry. -
Research • ChemistryPTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
Food Defense and Integrity
Food Preparation and Handling
Food Processing and Technology
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Education • Core: BiologyPlant Breeding and Biotechnology -
Education • Environmental SciencesTesting For Genetically Modified Foods -
Research • BiologyA Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling -
Research • Immunology and InfectionMonitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System -
Research • EnvironmentEcosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions -
Research • EnvironmentThe Effect of the Application of Thyme Essential Oil on Microbial Load During Meat Drying
Microbial Genetics
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Education • Microbiology16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species -
Research • BiologyGuided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing -
Education • Environmental MicrobiologyCommunity DNA Extraction from Bacterial Colonies -
Research • EnvironmentEnrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples -
Research • EnvironmentDetection of Foodborne Bacterial Pathogens from Individual Filth Flies -
Research • BioengineeringDevelopment of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen -
Research • EnvironmentTracking Microbial Contamination in Retail Environments Using Fluorescent Powder - A Retail Delicatessen Environment Example -
Research • Immunology and InfectionA High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
Microbiome
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Research • EnvironmentCharacterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities -
Research • Immunology and InfectionAn In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota -
Research • MedicineOral Biofilm Sampling for Microbiome Analysis in Healthy Children -
Research • GeneticsExploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere -
Research • EnvironmentA Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation
Natural Toxins
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Research • BiologyELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection -
Research • Immunology and InfectionQuantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays -
Research • ChemistryNatural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
Parasites
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Research • Immunology and InfectionOral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food -
Research • Immunology and InfectionDetermination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay -
Research • Immunology and InfectionA Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
Prevention and Control
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Research • BioengineeringThe Portable Chemical Sterilizer (PCS), D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military -
Research • EnvironmentColorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water -
Research • BiologyColorimetric Detection of Bacteria Using Litmus Test -
Research • Immunology and InfectionFoodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7 -
Research • Immunology and Infection'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens -
Research • Immunology and InfectionCombination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
Viruses and Prions
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Education • Core: BiologyRetrovirus Life Cycles -
Education • Core: BiologyViral Recombination -
Research • EnvironmentEPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples -
Research • EnvironmentEPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay -
Research • EnvironmentEPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR -
Research • Immunology and InfectionSwab Sampling Method for the Detection of Human Norovirus on Surfaces -
Research • Immunology and InfectionA Protocol for Analyzing Hepatitis C Virus Replication -
Research • Immunology and InfectionDevelopment of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle -
Research • Immunology and InfectionProcedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species
USDA Food Safety JoVE Publications
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Research • EnvironmentRNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem -
Research • BiologyA Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples -
Research • Immunology and InfectionGlass Wool Filters for Concentrating Waterborne Viruses and Agricultural Zoonotic Pathogens -
Research • EnvironmentInhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L. -
Research • EnvironmentControlled-release of Chlorine Dioxide in a Perforated Packaging System to Extend the Storage Life and Improve the Safety of Grape Tomatoes -
Research • EnvironmentProcedures of Laboratory Fumigation for Pest Control with Nitric Oxide Gas
