Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Murine Precision-Cut Liver Slices as an Ex Vivo…
JoVE Journal
Medicine
Author Produced

A subscription to JoVE is required to view this content.

JoVE Journal Medicine
Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology
Generation of Alginate Microspheres for Biomedical Applications
Journal (Medicine)
Generation of Alginate Microspheres for Biomedical Applications
Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses
Journal (Medicine)
Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses
 

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Article DOI: 10.3791/60992-v • 12:36 min • March 14th, 2020
March 14th, 2020 •
*1, *1, 2,3, 1,4,5, 6, 7, 8,9, 2,10, 1,4
1Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 3School of Veterinary and Life Sciences, Murdoch University, 4School of Medicine, The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine, Griffith University, 7School of Biological Sciences, Queen's University Belfast, 8Department of Gastroenterology & Hepatology, Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences, Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia
* These authors contributed equally

Chapters

Summary
Automatic Translation

This protocol provides a simple and reliable method for the production of viable precision-cut liver slices from mice. The ex vivo tissue samples can be maintained under laboratory tissue culture conditions for multiple days, providing a flexible model to examine liver pathobiology.

Tags

Precision-cut Liver Slices Ex Vivo Model Liver Biology Experimental Technique In Vivo Animal Studies In Vitro Cell Culture Control And Treatment Samples Isolation Of Liver Tissue Off-target Effects Toxic Pathway Inhibitors Vibrating Blade Shear Stress Viable Liver Tissue Laboratory Tissue Culture Bile Acids Cholestatic Liver Injury Hepatic Fibrogenesis
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter