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Murine Präzisionsgeschnittene Leberscheiben als Ex-Vivo-Modell der Leberbiologie
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Murine Präzisionsgeschnittene Leberscheiben als Ex-Vivo-Modell der Leberbiologie

Article DOI: 10.3791/60992-v • 12:36 min • March 14th, 2020
March 14th, 2020 •
*1, *1, 2,3, 1,4,5, 6, 7, 8,9, 2,10, 1,4
1Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 3School of Veterinary and Life Sciences, Murdoch University, 4School of Medicine, The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine, Griffith University, 7School of Biological Sciences, Queen's University Belfast, 8Department of Gastroenterology & Hepatology, Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences, Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia
* These authors contributed equally

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Dieses Protokoll bietet eine einfache und zuverlässige Methode für die Herstellung von lebensfähigen präzisionsgeschnittenen Leberscheiben von Mäusen. Die Ex-vivo-Gewebeproben können mehrere Tage lang unter Labor-Gewebekulturbedingungen aufbewahrt werden, was ein flexibles Modell zur Untersuchung der Leberpathobiologie bietet.

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Medizin Ausgabe 157 Leber Scheiben Vibratome ex vivo Hepatozyten Gewebe Kultur stellate Zellen
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