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JoVE Journal Neuroscience
Induktion af Leptomeningeal Celler Modifikation Via Intracisternal Injection
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Induktion af Leptomeningeal Celler Modifikation Via Intracisternal Injection

Article DOI: 10.3791/61009-v • 05:55 min • May 7th, 2020
May 7th, 2020 •
1, 1, 1
1Department of Cell and Molecular Biology, Karolinska Institute

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Vi beskriver en intracisternal injektion, der beskæftiger en nål bøjet på spidsen, der kan stabiliseres på kraniet, hvilket eliminerer risikoen for skader på den underliggende parenkym. Tilgangen kan bruges til genetisk skæbne kortlægning og manipulationer af leptomeningeal celler og til sporing cerebrospinalvæske bevægelse.

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Neurovidenskab Intracisternal injektion Leptomeninges Transgene mus Fate mapping Connexin-30 Cerebrospinalvæske Subarachnoid plads
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