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Biochemistry

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JoVE Journal Biochemistry
Detectie van eiwit s-acylation met behulp van Acyl-Hars Assisted Capture
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Detectie van eiwit s-acylation met behulp van Acyl-Hars Assisted Capture

Article DOI: 10.3791/61016-v • 08:31 min • April 10th, 2020
April 10th, 2020 •
*1, *1,2, 1, 1
1Department of Biochemistry and Molecular Biology, McGovern Medical School at UT Health, 2MD Anderson UT Health Graduate School
* These authors contributed equally

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Acyl-RAC (Acyl-Resin Assisted Capture) is een zeer gevoelige, betrouwbare en gemakkelijk uit te voeren methode om omkeerbare lipidemodificatie van cysteïneresiduen (S-acylation) in een verscheidenheid van biologische monsters op te sporen.

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Biochemie Nummer 158 Biochemie immunologie celbiologie S-acylation palmitoylation post-translationele modificatie Acyl-RAC splenocyten lymfocyten
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