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JoVE Journal
Biochemistry

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阿西尔-PEGyl交换凝胶移位测定,用于脑膜蛋白棕榈酸化定量测定
 
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阿西尔-PEGyl交换凝胶移位测定,用于脑膜蛋白棕榈酸化定量测定

Article DOI: 10.3791/61018-v 08:28 min March 29th, 2020
March 29th, 2020

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Summary

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棕榈化需要以可逆的方式将16碳棕榈酰化物与靶蛋白的半胱氨酸残留物结合。在这里,我们描述了一种生化方法,即acyl-PEGyl交换凝胶移位(APEGS)测定,以研究小鼠脑水化剂中任何感兴趣的蛋白质的棕榈酸化状态。

Tags

生物化学, 问题 157, 大脑, 突触, 棕榈化, AMPA 受体, SynDIG1, SynDIG4, Prrt1, Prrt2, APEGS 测定
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