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Biochemistry

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JoVE Journal Biochemistry
Acyl-PEGyl Exchange Gel Shift Analyse for kvantitativ bestemmelse av palmitoylering av hjernemembranproteiner
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Acyl-PEGyl Exchange Gel Shift Analyse for kvantitativ bestemmelse av palmitoylering av hjernemembranproteiner

Article DOI: 10.3791/61018-v • 08:28 min • March 29th, 2020
March 29th, 2020 •
1, 1
1Department of Pharmacology, UC Davis School of Medicine

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Palmitoylation innebærer inkorporering av en 16-karbon palmitat moiety til cystein rester av målproteiner på en reversibel måte. Her beskriver vi en biokjemisk tilnærming, acyl-PEGyl utveksling gel skift (APEGS) analyse, for å undersøke palmitoylering tilstand av ethvert protein av interesse for mus hjernen lysates.

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Biokjemi Utgave 157 hjerne synapse palmitoylering AMPA reseptor SynDIG1 SynDIG4 Prrt1 Prrt2 APEGS analyse
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