Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Acyl-PEGyl Exchange Gel Shift Analys för…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Acyl-PEGyl Exchange Gel Shift Analys för kvantitativ bestämning av palmitolerisering av hjärnans membranproteiner
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
Journal (Biochemistry)
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence
Journal (Biochemistry)
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence
 
Click here for the English version

Acyl-PEGyl Exchange Gel Shift Analys för kvantitativ bestämning av palmitolerisering av hjärnans membranproteiner

Article DOI: 10.3791/61018-v • 08:28 min • March 29th, 2020
March 29th, 2020 •
1, 1
1Department of Pharmacology, UC Davis School of Medicine

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Palmitolerisering innebär införlivande av en 16-karbon palmitat moiety till cysteinrester av målproteiner på ett reversibelt sätt. Här beskriver vi en biokemisk metod, acyl-PEGyl utbyte gel skift (APEGS) analys, att undersöka palmitoylering tillstånd av något protein av intresse för mus hjärnan lysates.

Tags

Biokemi Nummer 157 hjärna synaps palmitoylering AMPA-receptor SynDIG1 SynDIG4 Prrt1 Prrt2 APEGS-analys
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter