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Journal / Biochemistry / Beyin Zarı Proteinlerinin Palmitoylarasyonunun…
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Biochemistry

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JoVE Journal Biochemistry
Beyin Zarı Proteinlerinin Palmitoylarasyonunun Kantitatif Tayini için Acyl-PEGyl Exchange Jel Shift Tayini
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Beyin Zarı Proteinlerinin Palmitoylarasyonunun Kantitatif Tayini için Acyl-PEGyl Exchange Jel Shift Tayini

Article DOI: 10.3791/61018-v • 08:28 min • March 29th, 2020
March 29th, 2020 •
1, 1
1Department of Pharmacology, UC Davis School of Medicine

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Palmitoylation geri dönüşümlü bir şekilde hedef proteinlerin sistein artıkları için 16 karbon palmitate moiety dahil gerektirir. Burada, fare beyin lysates ilgi herhangi bir protein palmitoylation durumunu araştırmak için, bir biyokimyasal yaklaşım, asil-PEGyl değişim jel vardiya (APEGS) tsay açıklar.

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Biyokimya Sayı 157 beyin sinaps palmitoylation AMPA reseptörü SynDIG1 SynDIG4 Prrt1 Prrt2 APEGS tsnosu
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