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JoVE Journal Biochemistry
Acyl-PEGyl Exchange Gel Shift Assay zur quantitativen Bestimmung der Palmitoylierung von Gehirnmembranproteinen
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Acyl-PEGyl Exchange Gel Shift Assay zur quantitativen Bestimmung der Palmitoylierung von Gehirnmembranproteinen

Article DOI: 10.3791/61018-v • 08:28 min • March 29th, 2020
March 29th, 2020 •
1, 1
1Department of Pharmacology, UC Davis School of Medicine

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Die Palmitoylierung beinhaltet die Umarbeitung einer 16-Kohlenstoff-Palmitat-Moiety zu Cysteinrückständen von Zielproteinen in reversibler Weise. Hier beschreiben wir einen biochemischen Ansatz, den Acyl-PEGyl-Austausch-Gel-Shift (APEGS)-Assay, um den Palmitoylierungszustand eines Proteins zu untersuchen, das für Maushirnlysate von Interesse ist.

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Biochemie Ausgabe 157 Gehirn Synapse Palmitoylierung AMPA-Rezeptor SynDIG1 SynDIG4 Prrt1 Prrt2 APEGS-Assay
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