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Journal / Immunology and Infection / Ensayo de amplificación isotérmica mediada por…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Ensayo de amplificación isotérmica mediada por bucle de transcripción inversa (RT-LAMP) para la detección específica y rápida del virus del lago Tilapia
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Ensayo de amplificación isotérmica mediada por bucle de transcripción inversa (RT-LAMP) para la detección específica y rápida del virus del lago Tilapia

Article DOI: 10.3791/61025-v • 07:47 min • May 18th, 2020
May 18th, 2020 •
1, 2,3, 2, 4, 2,3
1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

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Presentamos un ensayo RT-LAMP para la detección de TiLV en peces de tilapia utilizando instrumentos simples durante un período de tiempo relativamente corto en comparación con las técnicas convencionales rt-PCR. Este protocolo puede ayudar a controlar la propagación epidémica de TiLVD, especialmente en los países en desarrollo.

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Inmunología e infección número 159 virus del lago de tilapia diagnóstico tilapia RT-LAMP RT-qPCR herramienta de detección
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