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Journal / Immunology and Infection / 反向转录循环介导的等温放大（RT-LAMP）测定，用于对罗非鱼湖病毒进行特定和快速检测…
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
反向转录循环介导的等温放大（RT-LAMP）测定，用于对罗非鱼湖病毒进行特定和快速检测
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反向转录循环介导的等温放大（RT-LAMP）测定，用于对罗非鱼湖病毒进行特定和快速检测

Article DOI: 10.3791/61025-v • 07:47 min • May 18th, 2020
May 18th, 2020 •
1, 2,3, 2, 4, 2,3
1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

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与传统RT-PCR技术相比，我们提出了一种RT-LAMP测定方法，用于在相对较短的时间内使用简单的仪器检测罗非鱼中的TiLV。该议定书可能有助于控制TiLVD的流行传播，特别是在发展中国家。

Tags

免疫学和感染， 问题 159， 罗非鱼湖病毒， 诊断， 罗非鱼， RT-LAMP， RT-qPCR， 筛查工具
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