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Immunology and Infection
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JoVE Journal Immunology and Infection
Ensaio de amplificação isotérmica mediada por loop de transcrição reversa (RT-LAMP) para a detecção específica e rápida do vírus do lago tilápia
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
Journal (Immunology and Infection)
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
Author Spotlight: Studying Macrophage-Epithelial Cell Interactions in Salivary Gland Regeneration After Injury
Journal (Immunology and Infection)
Author Spotlight: Studying Macrophage-Epithelial Cell Interactions in Salivary Gland Regeneration After Injury
 
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Ensaio de amplificação isotérmica mediada por loop de transcrição reversa (RT-LAMP) para a detecção específica e rápida do vírus do lago tilápia

Article DOI: 10.3791/61025-v • 07:47 min • May 18th, 2020
May 18th, 2020 •
1, 2,3, 2, 4, 2,3
1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

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Apresentamos um ensaio RT-LAMP para a detecção de TiLV em peixes de tilápia usando instrumentos simples durante um período relativamente curto de tempo em comparação com as técnicas convencionais de RT-PCR. Este protocolo pode ajudar a controlar a propagação epidêmica do TiLVD, especialmente nos países em desenvolvimento.

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Imunologia e Infecção Problema 159 vírus do lago tilápia diagnóstico tilápia RT-LAMP RT-qPCR ferramenta de triagem
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