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Metodi standardizzati per misurare l'induzione della risposta agli urti di calore in Caenorhabditis elegans
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Metodi standardizzati per misurare l'induzione della risposta agli urti di calore in Caenorhabditis elegans

Article DOI: 10.3791/61030-v • 06:01 min • July 3rd, 2020
July 3rd, 2020 •
1, 1, 1, 1
1Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology

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Qui, vengono presentati protocolli standardizzati per valutare l'induzione della risposta agli shock termici (HSR) in Caenorhabditis elegans utilizzando RT-qPCR a livello molecolare, reporter fluorescenti a livello cellulare e termorecupero a livello di organismo.

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Scienze Ambientali Numero 161 shock termico risposta agli urti termici HSF-1 C. elegans HSP-16.2 HSP-70 chaperones termotolleranza termorecupero
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