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JoVE Journal Developmental Biology
Fluorescerende immunolocalisatie van Arabinogalactan-eiwitten en pectines in de celwand van plantenweefsels
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Fluorescerende immunolocalisatie van Arabinogalactan-eiwitten en pectines in de celwand van plantenweefsels

Article DOI: 10.3791/61034-v • 10:14 min • February 27th, 2021
February 27th, 2021 •
1,2, 3, 1,2
1Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, 2Laboratório Associado para a Química Verde, 3Dipartimento di Bioscienze, Universitá degli Studi di Milano

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Dit protocol beschrijft in detail hoe het plantaardige materiaal voor immunolocalisatie van Arabinogalactan-eiwitten en pectines is bevestigd, ingebed in een hydrofiele acrylhars, doorsneden en gemonteerd op glazen dia's. We laten zien dat celwandgerelateerde epitopen worden gedetecteerd met specifieke antilichamen.

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Ontwikkelingsbiologie Arabinogalactan eiwitten celwanden Fluorescerende immunolocalisatie Histologie immunocytochemie LR-witte harsinbedding Pectines Plantencel Plantenweefsels
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