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JoVE Journal Developmental Biology
Fluorescerende immunlokalisering av arabinogalaktane proteiner og pektiner i celleveggen av plantevev
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Fluorescerende immunlokalisering av arabinogalaktane proteiner og pektiner i celleveggen av plantevev

Article DOI: 10.3791/61034-v • 10:14 min • February 27th, 2021
February 27th, 2021 •
1,2, 3, 1,2
1Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, 2Laboratório Associado para a Química Verde, 3Dipartimento di Bioscienze, Universitá degli Studi di Milano

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Denne protokollen beskriver i detalj hvordan plantematerialet for immunolokalisering av arabinogalaktane proteiner og pektiner er fast, innebygd i en hydrofil akrylharpiks, seksjonert og montert på glasssklier. Vi viser celleveggrelaterte epitoper vil bli oppdaget med spesifikke antistoffer.

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Utviklingsbiologi utgave 168 Arabinogalaktan proteiner cellevegger Fluorescerende immunolokalisering Histologi immunocytochemistry LR-hvit harpiks innebygging Pektiner Plantecelle Plantevev
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