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JoVE Journal Developmental Biology
Brug Af Flow Cytometry til at opdage og kvantificere ændret bloddannelse i den udviklende zebrafisk
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Brug Af Flow Cytometry til at opdage og kvantificere ændret bloddannelse i den udviklende zebrafisk

Article DOI: 10.3791/61035-v • 07:32 min • April 29th, 2021
April 29th, 2021 •
1, 1
1Department of Biological Sciences, California State University, Chico

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Denne analyse er en simpel metode til at kvantificere hæmatopoietiske celler i udviklingen af embryonale zebrafisk. Blodlegemer fra dissociated zebrafisk udsættes for flow cytometri analyse. Dette gør det muligt at opdage bloddefekter hos mutante dyr og efter genetisk modifikation.

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Udviklingsbiologi Problem 170 blodlegemer zebrafisk morfoer CRISPR hæmatopoiesis udvikling stamceller flowcytometry FACS
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