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Journal / Immunology and Infection / Produktion und Charakterisierung menschlicher…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Produktion und Charakterisierung menschlicher Makrophagen aus pluripotenten Stammzellen
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Produktion und Charakterisierung menschlicher Makrophagen aus pluripotenten Stammzellen

Article DOI: 10.3791/61038-v • 08:05 min • April 16th, 2020
April 16th, 2020 •
1,2, 1, 1, 1, 1, 2, 2, 1
1Centre for Regenerative Medicine, Scottish Centre for Regenerative Medicine, University of Edinburgh, 2Centre for Reproductive Health, The Queen's Medical Research Institute, University of Edinburgh

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Dieses Protokoll beschreibt die robuste Erzeugung von Makrophagen aus humaninduzierten pluripotenten Stammzellen und Methoden für deren anschließende Charakterisierung. Zelloberflächenmarkerexpression, Genexpression und funktionelle Assays werden verwendet, um den Phänotyp und die Funktion dieser iPSC-abgeleiteten Makrophagen zu bewerten.

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Immunologie und Infektion Ausgabe 158 pluripotente Stammzellen Differenzierung myeloische Zellen Makrophagen Phagozytose Polarisation
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