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JoVE Journal
Immunology and Infection

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简化反向遗传学方法,恢复重组性轮状病毒表达报告蛋白
 

简化反向遗传学方法,恢复重组性轮状病毒表达报告蛋白

Article doi: 10.3791/61039
April 17th, 2020

Summary April 17th, 2020

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从质粒DNA生成重组性轮状病毒为研究轮状病毒复制和发病机制以及发展轮状病毒表达载体和疫苗提供了重要工具。在这里,我们描述了一种简化的反向遗传学方法来产生重组性轮状病毒,包括表达荧光报告蛋白的菌株。

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