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Optimierung zur Sequenzierung und Analyse degradierter FFPE-RNA-Proben
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Optimierung zur Sequenzierung und Analyse degradierter FFPE-RNA-Proben

Article DOI: 10.3791/61060-v • 07:30 min • June 8th, 2020
June 8th, 2020 •
*1, *1,2, 1, 1, 1,2, 1
1NCI CCR Sequencing Facility, Frederick National Laboratory for Cancer Research, 2Advanced Biomedical and Computational Sciences, Frederick National Laboratory for Cancer Research
* These authors contributed equally

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Diese Methode beschreibt die Schritte zur Verbesserung der Qualität und Quantität der Sequenzdaten, die aus formal in-fixierten Paraffin-eingebetteten (FFPE) RNA-Proben gewonnen werden können. Wir beschreiben die Methodik zur genaueren Bewertung der Qualität von FFPE-RNA-Proben, bereiten Sequenzierungsbibliotheken vor und analysieren die Daten aus FFPE-RNA-Proben.

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Genetik Ausgabe 160 RNA-Sequenzierung formalinfixiertes Paraffin eingebettet FFPE Next Generation Sequenzierung NGS RNA-Seq-Analyse
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