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Journal / Genetics / 降解FFPE-RNA样品测序与分析优化
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JoVE Journal Genetics
降解FFPE-RNA样品测序与分析优化
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降解FFPE-RNA样品测序与分析优化

Article DOI: 10.3791/61060-v • 07:30 min • June 8th, 2020
June 8th, 2020 •
*1, *1,2, 1, 1, 1,2, 1
1NCI CCR Sequencing Facility, Frederick National Laboratory for Cancer Research, 2Advanced Biomedical and Computational Sciences, Frederick National Laboratory for Cancer Research
* These authors contributed equally

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该方法描述了提高从正式固定石蜡嵌入（FFPE）RNA样品中获得的序列数据的质量和数量的步骤。我们描述了更准确地评估FFPE-RNA样品质量、准备测序库和分析FFPE-RNA样品数据的方法。

Tags

遗传学， 问题 160， RNA 测序， 正式固定石蜡嵌入， FFPE， 下一代测序， NGS， RNA-seq 分析
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