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用于大型精子细胞三维共焦分析的全山 嗜血杆菌 睾丸免疫染色
JoVE Journal
Biology
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JoVE Journal Biology
Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes

用于大型精子细胞三维共焦分析的全山 嗜血杆菌 睾丸免疫染色

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08:37 min

August 27, 2020

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08:37 min
August 27, 2020

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Summary

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我们在这里提出了一个改进的协议,为全安装准备和免疫染色的 嗜血杆菌 睾丸,适合共焦显微镜,也允许可重复和可靠的标签。我们通过对大型S4-S5精子细胞的同位素化实验进行3D表示和量化来说明此协议。

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