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Immunostaining van Whole-Mount Drosophila Testes voor 3D Confocale Analyse van Grote Spermatocyten
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Immunostaining van Whole-Mount Drosophila Testes voor 3D Confocale Analyse van Grote Spermatocyten

Article DOI: 10.3791/61061-v • 08:37 min • August 27th, 2020
August 27th, 2020 •
1, 1, 1
1CNRS, Institut Jacques Monod, Université de Paris, F 75006, Paris, France

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We presenteren hier een verbeterd protocol voor de hele montering van drosophila-teelballen, geschikt voor confocale microscopie en ook reproduceerbaar en betrouwbaar labelen mogelijk. We illustreren dit protocol door 3D-representatie en kwantificering van colocalisatie-experimenten op de grote S4-S5 spermatocyten.

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Biologie Drosophila Melanogaster Teelballen Spermatocyten Immunofluorescentie Confocale microscopie Image 3D Colocalization Nucleoporin Nuclear Territory Nuclear pore complex
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