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Kartoffelvirus x-baseret microRNA-lyddæmpning (VbMS) i kartoffel.
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Kartoffelvirus x-baseret microRNA-lyddæmpning (VbMS) i kartoffel.

Article DOI: 10.3791/61067-v • 11:51 min • May 11th, 2020
May 11th, 2020 •
1, 1, 1,2
1Texas A&M AgriLife Research Center at Dallas, Texas A&M University System, 2Department of Plant Pathology, Microbiology, Texas A&M University

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Vi præsenterer en detaljeret protokol for kartoffelvirus X (PVX)-baserede microRNA silencing (VbMS) system til funktionelt karakterisere endogene mikroRNA'er (miRNAs) i kartoffel. Target mimic (TM) molekyler af miRNA af interesse er integreret i PVX vektor og forbigående udtrykt i kartoffel til tavshed målet miRNA eller miRNA familie.

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Miljøvidenskab udgave 159 virusbaseret microRNA-lyddæmpning (VbMS) kartoffelvirus X (PVX) microRNA (miRNA) target mimic (TM) kort tandemmål efterligner (STTMs) Solanum tuberosum
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