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Silenciador de microRNA baseado em vírus de batata (VbMS) na batata.
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Silenciador de microRNA baseado em vírus de batata (VbMS) na batata.

Article DOI: 10.3791/61067-v • 11:51 min • May 11th, 2020
May 11th, 2020 •
1, 1, 1,2
1Texas A&M AgriLife Research Center at Dallas, Texas A&M University System, 2Department of Plant Pathology, Microbiology, Texas A&M University

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Apresentamos um protocolo detalhado para o sistema de silenciamento de microRNA baseado em vírus de batata X (PVX) para caracterizar funcionalmente microRNAs endógenas (miRNAs) na batata. As moléculas de mímica alvo (TM) de miRNA de interesse são integradas ao vetor PVX e transitavelmente expressas na batata para silenciar a família miRNA ou miRNA alvo.

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Ciências Ambientais Questão 159 silenciamento de microRNA baseado em vírus (VbMS) vírus da batata X (PVX) microRNA (miRNA) mímica de alvo (TM) mímica de alvos de tandem curto (STTMs) tubos de solanum
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