Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / P. aeruginosa Co-culture 3D infectée des cellules …
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
P. aeruginosa Co-culture 3D infectée des cellules épithéliales bronchiques et des macrophages à l’interface air-liquide pour l’évaluation préclinique des anti-infectieux
Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Journal (Immunology and Infection)
Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
Journal (Immunology and Infection)
Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
 
Click here for the English version

P. aeruginosa Co-culture 3D infectée des cellules épithéliales bronchiques et des macrophages à l’interface air-liquide pour l’évaluation préclinique des anti-infectieux

Article DOI: 10.3791/61069-v • 10:26 min • June 15th, 2020
June 15th, 2020 •
*1,2, *1,2, 3, 4, 4, 3, 1, 1, 1,2
1Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy, Saarland University, 3Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, 4Department of Internal Medicine V - Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Nous décrivons un protocole pour un modèle tridimensionnel de co-culture des voies respiratoires infectées, utilisant CFBE41o- cellules, macrophages THP-1, et Pseudomonas aeruginosa, établi à l’interface air-liquide. Ce modèle fournit une nouvelle plate-forme pour tester simultanément l’efficacité antibiotique, la fonction de barrière épithéliale, et les marqueurs inflammatoires.

Tags

Ce mois-ci dans JoVE Numéro 160 Biofilms antibiotiques infections pulmonaires fibrose kystique inflammation interleukins TEER alternatives à l’expérimentation animale
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter