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Journal / Immunology and Infection / P. aeruginosa Co-Cultura 3D infectada de células…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
P. aeruginosa Co-Cultura 3D infectada de células epiteliais e macrófagos bronquiais na interface ar-líquida para avaliação pré-clínico de anti-infecciosos
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P. aeruginosa Co-Cultura 3D infectada de células epiteliais e macrófagos bronquiais na interface ar-líquida para avaliação pré-clínico de anti-infecciosos

Article DOI: 10.3791/61069-v • 10:26 min • June 15th, 2020
June 15th, 2020 •
*1,2, *1,2, 3, 4, 4, 3, 1, 1, 1,2
1Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy, Saarland University, 3Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, 4Department of Internal Medicine V - Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital
* These authors contributed equally

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Descrevemos um protocolo para um modelo tridimensional de co-cultura de vias aéreas infectadas, usando CFBE41o- células, macrófagos THP-1 e Pseudomonas aeruginosa, estabelecido na interface ar-líquido. Este modelo fornece uma nova plataforma para testar simultaneamente a eficácia dos antibióticos, a função da barreira epitelial e marcadores inflamatórios.

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Este mês em JoVE Edição 160 Biofilmes antibióticos infecções pulmonares fibrose cística inflamação interleucinas TEER alternativas aos testes em animais
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