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Journal / Cancer Research / 大肠癌HT29衍生癌症干细胞类肿瘤球中的驱动基因发现…
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JoVE Journal Cancer Research
大肠癌HT29衍生癌症干细胞类肿瘤球中的驱动基因发现
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大肠癌HT29衍生癌症干细胞类肿瘤球中的驱动基因发现

Article DOI: 10.3791/61077-v • 06:52 min • July 22nd, 2020
July 22nd, 2020 •
1, 4, 1, 1,2,3
1Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences, Chang Gung University, 3Department of Radiation Oncology, Chang Gung Memorial Hospital, 4Department of Laboratory Medicine, Mackay Memorial Hospital

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这里介绍的是一个协议，用于发现过度表达的驱动基因，维持从结肠直肠HT29细胞中提取的癌症干细胞样细胞。RNAseq 与可用的生物信息学执行调查和筛选基因表达网络，以阐明参与目标肿瘤细胞生存的潜在机制。

Tags

缩回， 问题 161， 结肠直肠癌， 癌症干细胞， CD133， HT29， LGR5， RNAseq， 网络分析
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