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JoVE Journal Cancer Research
Oppdagelsen av drivergener i kolorektal HT29-avledede kreftstammelignende tumorsfærer
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Oppdagelsen av drivergener i kolorektal HT29-avledede kreftstammelignende tumorsfærer

Article DOI: 10.3791/61077-v • 06:52 min • July 22nd, 2020
July 22nd, 2020 •
1, 4, 1, 1,2,3
1Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences, Chang Gung University, 3Department of Radiation Oncology, Chang Gung Memorial Hospital, 4Department of Laboratory Medicine, Mackay Memorial Hospital

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Presentert her er en protokoll for å oppdage de overuttrykte drivergenene som opprettholder de etablerte kreftstamlignende cellene avledet fra kolorektal HT29-celler. RNAseq med tilgjengelig bioinformatikk ble utført for å undersøke og screene genuttrykksnettverk for å belyse en potensiell mekanisme involvert i overlevelsen av målrettede tumorceller.

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Tilbaketrekking utgave 161 kolorektal kreft kreft stamcelle CD133 HT29 LGR5 RNAseq NetworkAnalyst
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